Examining The Inflammatory Effects of Woodsmoke on Murine Macrophages

Emily Brew

The purpose of this research was to determine the pro-inflammatory effects of woodsmoke on mouse macrophages. In developing countries, most of the population cooks indoors using stoves that require fire and they burn biomass. Our research uses woodsmoke to model the effects of indoor biomass smoke. When woodsmoke is inhaled it negatively impacts the immune system and causes inflammation in the lungs. Macrophages were treated with woodsmoke. We hypothesized that the woodsmoke would induce an inflammatory response and result in increased cytokine production. Zymosan (ZYM) and Lipopolysaccharide (LPS) are known activators of innate immunity and were used as positive controls. Several tests were used to evaluate cell death, cytokine production, and gene expression. Through the tests and assays we concluded that macrophages that were treated with a higher concentration of woodsmoke produced more cytokines, higher pro-inflammatory gene expression, and more cell death.

Department:

Biology

Mentor(s):

Shannon Jones