Examining the Effects of E-Cigarette Flavors at Different Concentrations on A549 Lung Epithelial Cells
This study investigates the inflammatory effects of e-cigarette flavors at different concentrations on human lung epithelial cells, A549 cells, to understand if differing ingredients cause different levels of toxicity. The flavors of ‘Mentholicious’, ‘Candy Cane’, and ‘Hed Rot’ were tested at various concentrations. The LDH Assay was used to measure the percentage of cells killed in a sample. To measure the concentration of IL-8 in the sample’s supernatant, an ELISA was performed. After using the sample’s RNA to make cDNA, a PCR and gel electrophoresis were run to amplify the amount of cDNA and separate the macromolecules by size. Cells treated with the compounds from the Candy Cane samples secreted more of the IL-8 protein during the ELISA’s than those treated with Mentholicious. There was also a trend found with the LDH Assays: the higher the concentrations any of the e-cigarette liquids resulted in a higher percentage of cell death.